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Salivary Fluoride Detection Kit: an indigenous way to diagnose human fluorosis


Background: Twenty states in India is currently suffering from fluorosis due to excess accumulation of fluoride. Literature reveals that excess fluoride concentration in the human body can cause urolithiasis (kidney stone). Therefore an easy-instant-economic sensor kit is highly essential for the detection of fluoride levels.

Challenge: As of now measurement of fluoride level in the human body is performed by testing blood specimens. Our target was to make the whole procedure easy and painless towards the measurement of fluoride concentration. We envisioned that if the salivary fluoride level could be measured then people will have an idea about the fluoride level which enters their bodies. Any food substance we consume first comes in contact with saliva; therefore measurement of salivary fluoride level could be the key factor towards quick detection of fluoride level which enters into the human body.

Achievement: To the best of our knowledge, for the first time CSIR-CMERI has prepared a salivary fluoride detection kit at an affordable cost. The diagnosis will be solely on human saliva however it should not be generalized as fluoride detection from water. The Salivary Fluoride detection kit will diagnose fluoride levels in saliva by colourimetric method. The provided colour scale has been developed after thorough standardization. Unlike costly-corrosive chemosensors, this chemosensor is low-cost, very easy to synthesize, non-toxic and non-corrosive in nature. Apart from the solution kit, a sensor station has been designed and fabricated by synchronization of colourimetric response with electronics. This sensor station could be deployed in any rural health centre or primary school as a kiosk for batch-scale analysis of saliva specimens.

Methodology: A very easy and consumer-friendly guideline has been provided with the kit by which any non-expertise can check the fluoride level in their own saliva. The methodology is shown here by the pictorial diagram. Initially, the patients have to spit in vial-1 and then add the solution from vial-2 into vial-1. Mix it and shake for 5 seconds. The obtained colour transition has to be then compared with the provided colour scale. The colour scale will indicate whether the patient is safe or unsafe in terms of fluoride level. After treatment, the vial-2 (containing saliva and chemosensor) has to be discarded in the sanitary pan. Soap paper is also supplied inside the kit for washing of hands after testing.

Ref: Indian patent granted on 25-05-2018, Patent number: IN201611039325


List of Patents
Patent Number Country Details
IN201611039325 India Salivary fluoride detection device and process of detection thereof
Priyabrata Banerjee; Pritam Ghosh; Naresh Chandra Murmu; Subhasis Biswas
IN201811019066 India A process for detection and measurement of fluoride level in water
Priyabrata Banerjee; Additi Roy Chowdhury; Asit Kumar Batabyal; Sourav Bej
IN201811022055 India Process for preparation of melamine-terephthaladehyde based covalent organic framework (mtcof) bio composite for spilled diesel clean up from water surface
Priyabrata Banerjee, Pritam Ghosh, Debanjan Dey, Naresh Chandra Murmu
IN201911013247 India Hospital solid waste (HSW) disposal plant utilizing electric arc plasma
Priyabrata Banerjee, Arabinda sarkar, Amit Ganguly, Partha Das, Harish Hirani
IN202111045215 India A combined mechanical and physico-chemical method for municipal sewage water treatment
Priyabrata Banerjee, Harish Hirani, Sourav Bej, Partha Das, Dipankar Chatterjee
IN202111051869 India A composition for high strength construction bricks utilizing fluoride contaminated sludge and a process for the preparation thereof
Asit Kumar Batabyal, Priyabrata Banerjee, Udayan Mondal, Somrita Nag, Harish Hirani
IN378228 India

A process for detection of fluoride level in an aqueous specimen with distinct colorimetric change
Priyabrata Banerjee, Pritam Ghosh, Suparna Paul, Asit Kumar Batabyal, Naresh Chandra Murmu

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